Journal: bioRxiv

Article Title: Characterising phagocytes and measuring phagocytosis from live Galleria mellonella larvae

doi: 10.1101/2023.10.09.558207

Figure Lengend Snippet: A) Scatter to demonstrate the increase of non-cellular events following centrifugation and fixation of splits of the same starting sample. The non-cellular gate is designated ‘debris’. B) Analysis of events in the debris gate using a cell membrane dye – CMG, and DAPI. Q1 represents CMG negative, DAPI positive, Q2 represents CMG positive, DAPI positive – therefore dead cells –, Q3 represents CMGreen positive, DAPI negative – therefore live cells –, and Q4 represents CMG negative, DAPI negative – true, non-cellular ‘debris’. In the filtered only sample, most events (69.6%) in the debris gate are deemed not cells (Q4). The profile dramatically changes following centrifugation – with more events becoming CMG positive (Q2 and Q3).

Article Snippet: A 400 μL aliquot of each sample was placed into a second 5 mL tube for staining with CellMask™ Green (CMG, ThermoFisher Scientific) at 1:2000 and/or DAPI (at 1:1000, ready-made solution, Merck).

Techniques: Centrifugation, Membrane

Journal: STAR Protocols

Article Title: An open access, machine learning pipeline for high-throughput quantification of cell morphology

doi: 10.1016/j.xpro.2022.101947

Figure Lengend Snippet:

Article Snippet: DAPI Ready Made Solution , Millipore Sigma , Cat # MBD0015.

Techniques: Recombinant, Software, Microscopy, Imaging